Fig. 4.

Blackett-Burman design of the different variables for production of Taxol by P. polonicum. Pareto Charts showing the effect of individual factors on Taxol production where: C- Dipotassium phosphate, F- Temperature, A- Sucrose, L- Peptone, E- Potassium chloride, B- Sodium nitrate, K- Malt extract, H- Incubation time, G- pH, J- shaking speed, D- Magnesium sulfate (A, B). The normal probability plots of the variables for Taxol production by P. polonicum as determined by the first order polynomial equation. C, Plot of correlation between predicted and actual Taxol yield by P. polonicum. D, Normal probability plots of residuals.