Table 7.
Face Centered Central Composite design of the variables; sucrose, dipotassium phosphate, potassium chloride and temperature on the yield of Taxol by P. polonicum.
| Runs | Sucrose (g/L) |
Dipotassium phosphate (g/L) |
Potassium chloride (g/L) |
Temperature (°C) |
Taxol yield (μg/l) |
||
|---|---|---|---|---|---|---|---|
| Experimental | Predicted | Residuals | |||||
| 1 | 40 | 2 | 1 | 30 | 332.08 | 371.0 | −38.89 |
| 2 | 40 | 2 | 0.5 | 25 | 313.76 | 355.1 | −41.38 |
| 3 | 35 | 1.50 | 0.75 | 22.50 | 238.14 | 262.0 | −23.81 |
| 4 | 30 | 2 | 1 | 25 | 180.24 | 144.2 | 36.05 |
| 5 | 40 | 2 | 1 | 25 | 229.2 | 206.3 | 22.92 |
| 6 | 40 | 2 | 0.5 | 30 | 239.04 | 234.3 | 4.78 |
| 7 | 30 | 1 | 0.5 | 25 | 55.68 | 54.6 | 1.11 |
| 8 | 30 | 2 | 1 | 30 | 231.6 | 254.8 | −23.16 |
| 9 | 25 | 1.5 | 0.75 | 27.5 | 143.28 | 107.5 | 35.82 |
| 10 | 40 | 1 | 1 | 25 | 146.16 | 127.2 | 19.00 |
| 11 | 35 | 2.5 | 0.75 | 27.5 | 211.44 | 232.6 | −21.14 |
| 12 | 35 | 1.5 | 0.75 | 32.5 | 194.96 | 191.1 | 3.90 |
| 13 | 35 | 1.5 | 0.75 | 27.5 | 169.92 | 186.9 | −16.99 |
| 14 | 40 | 1 | 1 | 30 | 204.0 | 239.0 | −35.0 |
| 15 | 35 | 1.5 | 1.25 | 27.5 | 189.5 | 208.5 | −18.95 |
| 16 | 35 | 1.5 | 0.75 | 27.5 | 172.2 | 146.3 | 25.82 |
| 17 | 35 | 1.5 | 0.75 | 27.5 | 174.6 | 144.9 | 29.68 |
| 18 | 30 | 1 | 1 | 30 | 151.9 | 127.6 | 24.31 |
| 19 | 45 | 1.5 | 0.75 | 27.5 | 194.6 | 190.7 | 3.89 |
| 20 | 30 | 2 | 0.50 | 25 | 138.8 | 138.8 | 0.00 |
| 21 | 35 | 1.5 | 0.75 | 27.5 | 169.3 | 147.3 | 22.01 |
| 22 | 35 | 1.5 | 0.75 | 27.5 | 169.4 | 203.3 | −33.89 |
| 23 | 40 | 1 | 0.50 | 30 | 156.1 | 187.3 | −31.22 |
| 24 | 35 | 0.5 | 0.75 | 27.5 | 151.8 | 182.1 | −30.35 |
| 25 | 40 | 1 | 0.50 | 25 | 104.6 | 125.6 | −20.93 |
| 26 | 35 | 1.5 | 0.75 | 27.5 | 169.0 | 202.8 | −33.81 |
| 27 | 30 | 1 | 0.50 | 30 | 107.0 | 107.0 | 0.00 |
| 28 | 30 | 1 | 1 | 25 | 99.4 | 119.3 | −19.89 |
| 29 | 35 | 1.5 | 0.25 | 27.50 | 150.8 | 165.9 | −15.08 |
| 30 | 30 | 2 | 0.50 | 30 | 221.3 | 190.3 | 30.98 |