Table 2.

Effect of supplementing BSA to Lake and ASG extenders on rooster semen quality.

BSA:	−		+		BSA		Extender			Significance
Extender:	Lake	ASG	Lake	ASG	−	+	Lake	ASG	SEM	BSA (A)	Extender (B)	A×B
FRESH SEMEN
Viable spermatozoa (%)	80.50A(2)	71.50B(2)	78.50	77.60	76.00	78.05	79.50a	74.55b	1.34	NS	*	*(2)
Motility quality score (1-5)1	2.72	2.85	3.97	3.95	2.78b	3.96a	3.35	3.40	0.10	*	NS	NS
Non-progressive sperm (%)	37.62	34.69	16.74	27.92	36.15a	22.33b	27.18	31.30	2.47	*	NS	NS
Progressive sperm (%)	31.52	23.66	55.92	46.50	27.59b	51.21a	43.72	35.08	3.15	*	NS	NS
Total motility (%)	69.14	58.35	72.67	74.42	63.75	73.54	70.90	66.39	3.61	NS	NS	NS
VCL (µm/s)	75.39	68.61	116.69	100.88	72.00b	108.78a	96.04	84.74	4.41	*	NS	NS
VSL (µm/s)	45.20	35.25	92.59	75.80	40.23b	84.20a	68.89a	55.53b	4.39	*	*	NS
VAP (µm/s)	55.45	47.00	102.11	85.91	51.22b	94.01a	78.78	66.45	4.29	*	NS	NS
LIN (%)	57.27	50.73	78.80	74.24	54.00b	76.52a	68.03	62.48	2.37	*	NS	NS
STR (%)	78.88	73.51	90.36	87.46	76.20b	88.91a	84.62	80.49	1.62	*	NS	NS
WOB (%)	72.01	68.18	87.03	84.67	70.09b	85.85a	79.52	76.42	1.46	*	NS	NS
ALH (µm)	2.97	2.92	3.06	3.28	2.95	3.17	3.02	3.10	0.11	NS	NS	NS
BCF (Hz)	9.60	8.78	10.28	9.82	9.19b	10.05a	9.94	9.30	0.24	*	NS	NS
POST-THAW SEMEN
Viable spermatozoa (%)	36.90	30.30	33.90B(3)	43.40A(3)	33.60b	38.65a	35.40	36.85	1.71	*	NS	*(3)
Motility quality score (1-5)1	1.47	1.95	2.70	2.72	1.71b	2.71a	2.08	2.33	0.14	*	NS	NS
Non-progressive sperm (%)	12.49	14.04	15.65	20.50	13.26b	18.08a	14.07	17.27	1.37	*	NS	NS
Progressive sperm (%)	5.39	9.48	16.58	15.28	7.43b	15.93a	10.99	12.38	1.23	*	NS	NS
Total motility (%)	17.88	23.52	32.24	35.78	20.70b	34.01a	25.06	29.65	2.24	*	NS	NS
VCL (µm/s)	48.22B(4)	61.76A(4)	67.90	61.84	54.99b	64.87a	58.06	61.80	2.61	*	NS	*(4)
VSL (µm/s)	28.42B(5)	39.07A(5)	50.19	45.83	33.74b	48.01a	39.30	42.45	2.45	*	NS	*(5)
VAP (µm/s)	34.68B(6)	47.08A(6)	56.32	51.70	40.88b	54.01a	45.50	49.39	2.55	*	NS	*(6)
LIN (%)	58.13	62.62	73.18	73.64	60.38b	73.41a	65.66	68.13	1.79	*	NS	NS
STR (%)	81.07	82.45	88.68	88.43	81.76b	88.55a	84.88	85.44	1.11	*	NS	NS
WOB (%)	71.42	75.68	82.30	83.15	73.55b	82.73a	76.86	79.42	1.25	*	NS	NS
ALH (µm)	2.16B(7)	2.74A(7)	2.59	2.39	2.45	2.49	2.38	2.57	0.09	NS	NS	*(7)
BCF (Hz)	8.84	9.14	9.55	9.20	8.99	9.37	9.19	9.17	0.26	NS	NS	NS

(-) Absence of BSA, (+) Supplementation with BSA

Abbreviations: Lake, Lake and Ravie 1984 medium; ASG, Animal Sciences Group (Wageningen University, Lelystad, The Netherlands) medium; NS, non-significant differences; VCL, curvilinear velocity; VSL, straight-line velocity; VAP, average path velocity; LIN, linearity; STR, straightness; WOB, wobble; ALH, amplitude of lateral head displacement; BCF, beat-cross frequency.

^a,bMeans within a row with no common superscript differ significantly (*P < 0.05)

¹Subjective measurement: The quality of motility was scored on a scale of 0 (lowest) to 5 (highest); 0 = no movement, 1 = tail movements but no sperm progression, 2 = only circular sperm movements, 3 = a large percentage of spermatozoa showed progressive but no rectilinear movement, 4 = a large percentage of spermatozoa showed rectilinear but not very vigorous movement, and 5 = a large percentage of spermatozoa showed vigorous, rectilinear, progressive movement.

^2(A,B)Interaction effect (BSA×Extender) for viable spermatozoa (%) in fresh semen.

^3(A,B)Interaction effect (BSA×Extender) for viable spermatozoa (%) in post-thaw semen.

^4(A,B)Interaction effect (BSA×Extender) for VCL (µm/s) in post-thaw semen.

^5(A,B)Interaction effect (BSA×Extender) for VSL (µm/s) in post-thaw semen.

^6(A,B)Interaction effect (BSA×Extender) for VAP (µm/s) in post-thaw semen.

^7(A,B)Interaction effect (BSA×Extender) for ALH (µm) in post-thaw semen.