Table 3.
Significant CpGs associated with alcohol consumption in a within-pair epigenome-wide analysis of WBCs from the FinnTwin cohort.
| CpG | UCSC Gene | Chr | Position | P value | FDR adjusted P value | β | SE | Relation to UCSC CpG Island | Enhancer |
|---|---|---|---|---|---|---|---|---|---|
| cg17625416 | ZMYM2 | 13 | 20533511 | 4.42E-14 | 1.86E-08 | −0.00142 | 1.89E-04 | Island | |
| cg02208313 | CCDC57 | 17 | 80085332 | 4.39E-12 | 9.26E-07 | −0.00252 | 3.64E-04 | Island | |
| cg13232357 | GABPB2 | 1 | 151042823 | 1.05E-11 | 1.47E-06 | −0.00236 | 3.48E-04 | N_Shore | |
| cg13319698 | 11 | 66176589 | 1.77E-11 | 1.70E-06 | 0.00065 | 9.72E-05 | Island | ||
| cg15083677 | 6 | 164758516 | 2.01E-11 | 1.70E-06 | 0.00265 | 3.95E-04 | S_Shore | ||
| cg11404486 | GOLGA8B | 15 | 34875930 | 2.87E-11 | 1.87E-06 | 0.00181 | 2.72E-04 | Island | |
| cg22396353 | GTSE1 | 22 | 46692687 | 3.10E-11 | 1.87E-06 | −0.00175 | 2.63E-04 | Island | |
| cg08758185 | SLC36A1 | 5 | 150849936 | 6.10E-11 | 3.22E-06 | 0.00257 | 3.92E-04 | OpenSea | TRUE |
| cg21851395 | 13 | 42108056 | 7.84E-11 | 3.67E-06 | −0.00256 | 3.94E-04 | OpenSea | TRUE | |
| cg20126775 | DEFB133 | 6 | 49917145 | 1.06E-10 | 4.46E-06 | −0.00237 | 3.67E-04 | OpenSea | |
| cg10177748 | HNRNPUL1 | 19 | 41770160 | 2.72E-10 | 1.04E-05 | −0.00065 | 1.02E-04 | Island | |
| cg17366225 | 4 | 11398983 | 7.68E-10 | 2.70E-05 | 0.00203 | 3.29E-04 | N_Shore | ||
| cg14863944 | 13 | 22740131 | 1.48E-09 | 4.47E-05 | 0.00244 | 4.03E-04 | OpenSea | ||
| cg24650913 | B3GNTL1 | 17 | 80970434 | 1.43E-09 | 4.47E-05 | 0.00244 | 4.03E-04 | S_Shore | |
| cg03180302 | TTR | 18 | 29171878 | 3.11E-09 | 8.74E-05 | 0.00226 | 3.81E-04 | OpenSea | |
| cg00699945 | NKX6–2 | 10 | 134600651 | 4.74E-09 | 1.25E-04 | −0.00020 | 3.44E-05 | Island | |
| cg13490575 | C9orf3 | 9 | 97735830 | 8.26E-09 | 2.05E-04 | 0.00227 | 3.94E-04 | OpenSea | TRUE |
| cg02583546 | C14orf4 | 14 | 77494451 | 9.84E-09 | 2.31E-04 | −0.00070 | 1.22E-04 | Island | |
| cg09168036 | MRPL13 | 8 | 121457072 | 2.45E-08 | 5.44E-04 | −0.00099 | 1.77E-04 | N_Shore | |
| cg18760251 | SEC62 | 3 | 169684683 | 5.93E-08 | 1.25E-03 | −0.00010 | 1.87E-05 | Island | |
| cg13496066 | TMEM231 | 16 | 75580831 | 8.13E-08 | 1.63E-03 | 0.00164 | 3.06E-04 | OpenSea | |
| cg16207974 | SLMO1 | 18 | 12431241 | 2.16E-07 | 4.13E-03 | 0.00037 | 7.05E-05 | Island | |
| cg08859215 | FAM20A | 17 | 66573573 | 3.37E-07 | 6.18E-03 | −0.00187 | 3.67E-04 | OpenSea | TRUE |
| cg07993036 | 22 | 46467577 | 6.75E-07 | 1.19E-02 | −0.00033 | 6.72E-05 | Island | ||
| cg18752774 | NLRP14 | 11 | 7060500 | 1.04E-06 | 1.75E-02 | 0.00249 | 5.11E-04 | OpenSea | |
| cg07886701 | FABP9 | 8 | 82375121 | 2.28E-06 | 3.57E-02 | 0.00212 | 4.48E-04 | OpenSea | |
| cg13620744 | 19 | 36478289 | 2.27E-06 | 3.57E-02 | 0.00015 | 3.10E-05 | Island |
Abbreviations: WBCs, whole blood cells; CpG, cytosine-phosphate-guanine dinucleotide; Chr, Chromosome; SE, standard error.
Notes. Within-pair epigenome-wide association of continuous alcohol consumption was performed using whole blood cell-derived DNA samples from the Finnish Twin Cohort. Within-pair differences were calculated and fit to a linear model, with DNA methylation proportion difference as the outcome variable and difference in alcoholic drinks consumed per week as the predictor variable. Within-pair differences in BMI, smoking status, and white blood cell counts were adjusted, and CpG loci were annotated to the human reference genome release GRCh37 with the “IlluminaHumanMethylation450kanno.ilmn12.hg19” R package.