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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Pharm Dev Technol. 2020 Feb 12;25(5):625–639. doi: 10.1080/10837450.2020.1725893

Table 2.

Mobile phase gradient for LCMS method

Time (min) 0.1% formic acid in water (%A) 0.1% formic acid in acetonitrile (%B)
0.01 30 70
2.50 30 70
2.50 2 98
3.50 2 98
3.51 30 70
5.00 30 70