Figure 8.

CCT2 overexpression promotes progression of breast cancer cells through the G1/S transition. Proliferation of T47D and MCF7 cells, CCT2-FLAG overexpressing and lentiviral control, in 2D cultures was synchronized after serum deprivation for 24 hours and cell cycle distribution was analyzed by PI intracellular staining of cells from 2D monolayer cultures (A, B) and 2D post 3D cultures (C, D) after 24 and 48 hours of serum introduction (n=3). Note that ~70% of the cells were synchronized in G1 after serum deprivation ( Figure S7 ). Data was acquired using the CytoFlex S flow cytometer and analyzed with FCS Express software.