FIGURE 7.

Effect of RUP with or without wortmannin on ISO-induced histological changes. Normal group (A,F,K), ISO group (B,G,L), RUP group (C,H,M) and RUP + Wort group (D,I,N). (A–D) Specimens stained with Masson’s trichrome for estimation of myocardial fibrosis (blue color). Specimens stained with hematoxylin and eosin for estimation of degree of myocardial damage (F–I) and hypertrophy (K–N). Percentage of fibrosis (E) and left ventricular (LV) thickness (O). Each value represents the mean of 5 experiments and error bars represent SD. Statistical analysis was done using One way ANOVA followed by Tukey’s post-hoc test. Histopathological scores (J) are expressed as the median of 5 animals. Statistical analysis was done using Kruskal-Wallis test followed by Dunn’s test where a p < 0.05 vs. normal, b p < 0.05 vs. ISO, c p < 0.05 vs. RUP.