TABLE 5.
Linum usitatissimum genes encoding proteins with lectin domains upregulated in flax stem tissue samples with the tertiary cell wall.
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A pairwise comparison (log2FC ≥ 1, padj ≥ 0.01) was made with tissue samples that deposit primary and secondary cell walls. The gene description includes the gene ID according to Phytozome v12.1.6 (https://phytozome.jgi.doe.gov/pz/portal.html; Goodstein et al., 2012); the lectin family name that was used in present study; the Pfam domain by the presence of which lectin family members were distinguished; the gene description according to Phytozome v12.1.6; Arabidopsis thaliana homolog for a flax gene according to flax genome annotation (Wang et al., 2012); subcellular localization of A. thaliana homologs for flax lectins according to the SUBA4 database (https://suba.live/; Hooper et al., 2017); and subcellular localization of flax lectins (corrected protein sequences presented in Supplementary File 1 were used) according to the LocTree3 prediction tool (https://rostlab.org/services/loctree3/; Goldberg et al., 2014). The red-blue heat map indicates that the mRNA abundance expressed in TGR (total gene reads) values and the color gradient reflects TGR values of 1 to 1000. Abbreviations: SAM, shoot apical meristem; cPAR, cortical parenchyma; iFIB, phloem fibers at the stage of intrusive elongation; sXYL, stem xylem with secondary cell wall; tFIB, phloem fibers depositing tertiary cell walls; PM, plasma membrane.