FIGURE 1.

Scheme of priming, challenge, and sampling for RNA-seq. Newly emerged An. gambiae G3 mosquitoes were set into three cohorts for priming treatment via sugar meal: native microbiota, supplemented with Enterobacter and Serratia. The primed cohorts were then subject to Enterobacter and Serratia challenge, respectively. The sterile H₂O injection in native primed cohort was used as injury control. RNA samples were extracted from each cohort for RNA-seq, a total of 30 samples were collected from 10 conditions with three replicates for each condition.