Figure 6.

Hippocampal and cortical Zbtb20 expression in adult hAPP-J20 mice and NTG controls. A, Representative confocal photomicrographs of coronal dentate gyrus sections from four- to five-month-old NTG and hAPP-J20 mice immunostained for Zbtb20 (scale bar: 200 μm). B, Relative Zbtb20 immunoreactivity (IR; signal intensity) in the granular layer of the dentate gyrus in four- to five-month-old NTG and hAPP-J20 mice. Mean signals in NTG mice were defined as 1.0. C–F, Zbtb20 mRNA and protein levels in the hippocampus and cortex of six-month-old NTG and hAPP-J20 mice were determined by RT-qPCR and western blot analysis, respectively, as in Figure 3. G,H, Zbtb20 mRNA levels in the hippocampus and cortex of six- to seven-month-old NTG and APP/PS1 mice determined by RT-qPCR; n = 4–6 male and 5–8 female mice per group; *p < 0.05, ***p < 0.001, ****p < 0.0001 by unpaired two-tailed Student’s t test. ns, not significant. Dots represent individual mice and bars are means ± SEM.