Fig. 1. Cryo-EM reconstruction of BinJV.
(A) SDS-PAGE analysis of gradient purified BinJV, HVV, PaRV, and WNVKUN virions. Virus preparations are nonreduced and stained with SYPRO Ruby stain. E, prM, capsid (C), and M proteins indicated by arrows. (B) Surface cryo-EM reconstruction of BinJV. Density attributed to the pr proteins within the immature form is shown in yellow, and the three E proteins that make up the asymmetric unit were shown in shades of green. Ellipses, triangles, and pentagons represent the two-, three-, and fivefold icosahedral axes, respectively. (C) The asymmetric unit of BinJV is composed of three copies of E (green, pink, and cyan) and prM (dark green, magenta, and blue). (D) Side view of the asymmetric unit displayed as a cartoon and colored by symmetry position. Density is shown for prM. Lines illustrate the extent of the lipid bilayer.