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. 2021 May 14;7(20):eabe4507. doi: 10.1126/sciadv.abe4507

Fig. 7. 2A7 blocks in vitro maturation in an isotype-dependent manner.

Fig. 7

(A) Purified BinJV was incubated with anti-prM 2A7 antibodies (IgM, IgG, and Fab) or the control antibody CO5 (IgM and IgG) for 1 hour before incubating with furin and analyzing by SYPRO Ruby–stained SDS-PAGE. Virus and antibodies were mixed at equimolar concentrations. Viral proteins are indicated by arrows, and antibody fragments are in brackets. The relative intensity of the prM (B) and M (C) bands following incubation with and without furin was quantified. n = 2 independent experiments; means ± SD. (D) Model of neutralization of immature BinJV by the 2A7-IgM. Schematic representation of five prM-E trimers that are presented in gray (E) and blue (pr) surrounding the fivefold axis. Multimeric binding of IgM antibody are proposed to cross-link the prM-E spikes inhibiting low pH–mediated release of the furin site during endocytosis. Distance between antigen binding sites of IgM (51) and IgG (52) antibody isotypes and the span across the fivefold axis is annotated.