Fig. 5. Immune phenotypes of pMHC binding CD8⁺ T cells.

(A) Classification of pMHC binding cells. (B) Heatmap of the expression of CD8⁺ T cell subtype marker genes and proteins. *: protein expression measured by CITE-seq. (C) pMHC binding landscape by CD8⁺ T cell immune subtypes. Bars indicate the number of pMHC binding T cells in log₂ scale. (D) Expanded clonotypes are enriched in the non-naïve compartment. Each dot represents a unique TCR clone. (E) Pie chart of subpopulations of pMHC binding CD8⁺ T cells. (F) Fraction of HLA type matched and mismatched binding naïve and non-naïve T cells. T_pm, peripheral memory cells; T_cm, central memory cells; T_em, effector memory cells; T_emra, terminally differentiated effector memory cells; Others, other memory cells with the marker gene expression pattern CD43^loCD127^loKLRG1^high.