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. 2021 May 14;7(20):eabg4123. doi: 10.1126/sciadv.abg4123

Fig. 3. In vitro setup and analysis of myogenic behavior.

Fig. 3

(A) C2C12 myoblasts were allowed to attach to dECM scaffolds while being incubated overnight on a rotating system. (B) After seeding, cell-laden scaffolds were collected and cultured in stationary plates in growth media for 4 days. (C) At 2 and 4 days in growth media, cell growth was assessed. (D) After 4 days, growth media was swapped for induction media. Cells were then allowed to differentiate for 7 days. (E) Myotube formation was then assessed on cell-laden scaffolds.