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. 2021 May 17;131(10):e146614. doi: 10.1172/JCI146614

Figure 3. Junctional diversity in MIS-C patients with TRBV11-2 expansions.

Figure 3

(A) Overlap of CDR3 amino acid clonotypes per 200 sequences in repetitive samples of a healthy individual and between repertoires of MIS-C patients with TRBV11-2 expansion. (B) PCA and differential usage of J genes rearranged with TRBV11-2 in MIS-C patients and febrile controls. Bars indicate mean ± SD. Statistical analysis: Pillai-Bartlett test in MANOVA of all principal components. (C) Heatmap of productive CDR3 length distribution in the repertoires of MIS-C patients (n = 20) and febrile controls (n = 15). (D) CDR3 diversity in MIS-C patients with or without expanded TRBV11-2 and febrile controls displayed as positional weight matrix generated using GLAM2. (E) Unsupervised phylogenetic analysis of the amino acid sequences spanning FR2 to CDR3 versus CDR3 alone in the top 100 clones of MIS-C repertoires (n = 20), either comprising the complete TRBV sequence pool or TRBV11-2 sequences only.