Figure 2. ATT-I enhances the antigen-specific T cell responses in MC38 tumor–derived organoids.
(A) Schematic representation of the MC38-OVA–derived organoid killing assay. OT-I CD8+ T cells were cocultured with tumor organoids generated from MC38-derived tumors in C57BL/6 mice with or without ATT-I treatment. (B) Representative figures of MC38-derived tumor organoids taken at the indicated timepoints from the same well with control or ATT-I treatment. Scale bar: 100 μm. (C) Quantification of the organoid size presented as mean ± SD of 3 parallel experiments. The size of organoids was measured as project area (μm2) using Image J software. Statistical analysis was conducted using 2-way ANOVA. **P < 0.01; ***P < 0.001; ****P < 0.0001.