Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) | Il33 | GenBank | MGI:1924375 | https://www.ncbi.nlm.nih.gov/gene/77215 |
| Gene (Mus musculus) | Il1rl1 | GenBank | MGI:98427 | https://www.ncbi.nlm.nih.gov/gene/17082 |
| Strain, strain background (Mus musculus) | C57BL/6NTac | Taconic | RRID:MGI:5658006 | |
| Genetic reagent (Mus musculus) |
B6.129S7-Rag1tm1Mom/J | Jackson | RRID:IMSR_JAX:002216 | |
| Genetic reagent (Mus musculus) |
C57BL/6NTac.Rag2tm1Fwa;II2rgtm1Wjl | Taconic | Cat # 4111 | |
| Genetic reagent (Mus musculus) |
B6(129S4)-Il33tm1.1Bryc/J | Jackson | RRID:IMSR_JAX:030619 | |
| Genetic reagent (Mus musculus) |
Il1rl1tm1Anjm | PMID:10727469 Townsend et al., 2000 | MGI:2386675 | http://www.informatics.jax.org/allele/MGI:2386675 |
| Strain, strain background (Toxoplasma gondii) | ME49 | NCBI:txid508771 | ||
| Strain, strain background (Toxoplasma gondii) | Pru-tdTomato | PMID:19578440 John et al., 2009 | ||
| Strain, strain background (Toxoplasma gondii) | CPS | PMID:11859373 Fox and Bzik, 2002 | ||
| Antibody |
Toxoplasma gondii
Rabbit polyclonal |
Collaborator | IHC: 1:100 | |
| Antibody | iNOS Rabbit polyclonal |
Abcam | Cat # ab15323, RRID:AB_301857 | IHC 1:50 |
| Antibody | ERTR7 Af647 Rat monoclonal (sc-73355) |
Santa Cruz Biotechnology | Cat # sc-73355 RRID:AB_1122890 |
IF (1:50) |
| Antibody | F4/80 BV480 Rat monoclonal (T45-2342) |
BD | Cat # 565635 RRID:AB_2739313 |
IF (1:25) |
| Antibody | CD45 Af700 Rat monoclonal (30-F11) |
BioLegend | Cat # 103127, RRID:AB_493714 | IF (1:20) |
| Antibody | CD335 NKp46 PE/Dazzle 594 Rat monoclonal (29A1.4) |
BioLegend | Cat # 137629, RRID:AB_2616665 | FC (1:200) |
| Antibody | NK-1.1 BV711 Mouse monoclonal (PK136) |
BioLegend | Cat # 108745, RRID:AB_2563286 | FC (1:200) |
| Antibody | IFN gamma Af700 Rat monoclonal (XMG1.2) |
Thermo Fisher | Cat # 56-7311-82, RRID:AB_2688063 | FC (1:200) |
| Antibody | CD200 Receptor APC Rat monoclonal (OX110) |
Thermo Fisher | Cat # 17-5201-82, RRID:AB_10717289 | FC (1:200) |
| Antibody | T1/ST2 Biotin Rat monoclonal (DJ8) |
MD Biosciences | Cat # 101001B, RRID:AB_947551 | FC (1:200) |
| Antibody | T-bet PE-Cy7 Mouse monoclonal (4B10) |
BioLegend | Cat # 644823 | FC (1:200) |
| Antibody | EOMES PE Rat monoclonal (Dan11mag) |
Thermo Fisher | Cat # 12-4875-82, RRID:AB_1603275 | FC (1:200) |
| Antibody | CD11b ef450 Rat monoclonal (M1/70) |
Thermo Fisher | Cat # 48-0112-80, RRID:AB_1582237 | FC (1:1000) |
| Antibody | CD11c APC-ef780 Armenian hamster monoclonal (N418) |
Thermo Fisher | Cat# 47-0114-80, RRID:AB_1548663 | FC (1:200) |
| Antibody | Ly-6C BV785 Rat monoclonal (HK1.4) |
BioLegend | Cat # 128041, RRID:AB_2565852 | FC (1:200) |
| Antibody | Ly-6G BV711 Rat monoclonal (1A8) |
BioLegend | Cat # 127643, RRID:AB_2565971 | FC (1:200) |
| Antibody | CCR2 CD192 APC Rat monoclonal (SA203G11) |
BioLegend | Cat # 150628, RRID:AB_2810415 | FC (1:200) |
| Antibody | CD64 FcgammaRI PE-Cy7 Mouse monoclonal (X54-5/7.1) |
BioLegend | Cat # 139306, RRID:AB_11219391 | FC (1:200) |
| Antibody | MHC Class II (I-A/I-E) Af700 Rat monoclonal (M5/114.15.2) |
Thermo Fisher | Cat # 56-5321-82, RRID:AB_494009 | FC (1:200) |
| Antibody | iNOS APC Rat monoclonal (CXNFT) |
Thermo Fisher | Cat # 17-5920-82, RRID:AB_2573244 | FC (1:200) |
| Antibody | Podoplanin gp38 PerCP-ef710 Syrian hamster monoclonal (eBio8.1.1) |
Thermo Fisher | Cat # 46-5381-82, RRID:AB_2848339 | FC (1:200) |
| Antibody | CD31 BV605 Rat monoclonal (390) |
BioLegend | Cat # 102427, RRID:AB_2563982 | FC (1:200) |
| Peptide, recombinant protein | Recombinant murine IL-33 | Peprotech | Cat # 210–33 | |
| Commercial assay or kit | IL-33 ELISA | R and D Biosystems | Cat # DY3626 |