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. 2021 May 11;12(10):1011–1023. doi: 10.18632/oncotarget.27954

Table 3. Linear and circular transcripts of TRAM1 and S100A6.

(A) TRAM1 (translocation associated membrane protein 1)
Transcript ID Name length [kb] HR [TPM] C [TPM] log(HR/C)
hsa_circ_0084758 TRAM1 0.176 0.66 41.33 –1.80
hsa_circ_0084756 TRAM1 0.383 0.81 21.56 –1.43
hsa_circ_0084757 TRAM1 0.447 0.98 18.51 –1.28
hsa_circ_0084759 TRAM1 0.422 3.08 20.04 –0.81
- - 1.38 25.36 –1.26
Transcript ID Name length [kb] HR [TPM] C [TPM] log(HR/C) TSL Biotype
ENST00000520700 TRAM1-203 0.542 1.630 20.115 –1.091 5 Pt
ENST00000521425 TRAM1-205 3.394 5.126 8.141 –0.201 2 Pc
ENST00000262213 TRAM1-201 2.785 6.077 8.431 –0.142 1 Pc
ENST00000521049 TRAM1-204 0.872 3.700 2.805 0.120 5 Pt
ENST00000518678 TRAM1-202 0.535 4.339 3.043 0.154 4 Pc
- - 4.28 12.23 –0.46 - -
(B) S100A6 (S100 Calcium Binding Protein 6)
Transcript ID Name length [kb] HR [TPM] C [TPM] log(HR/C)
hsa_circ_0014225 S100A6 0.390 391.17 102.25 0.58
hsa_circ_0014226 S100A6 0.683 314.28 87.06 0.56
hsa_circ_0014224 S100A6 0.231 105.43 29.96 0.55
hsa_circ_0014227 S100A6 0.452 21.51 7.32 0.47
- - 352.73 94.66 0.57
Transcript ID Name length [kb] HR [TPM] C [TPM] log(HR/C) TSL Biotype
ENST00000496817 S100A6-205 0.673 79.051 19.216 0.614 2 Pc
ENST00000462951 S100A6-204 0.311 10.455 4.698 0.347 1 Ri
ENST00000368720 S100A6-202 0.673 70.632 17.232 0.613 3 Pc
ENST00000368719 S100A6-201 0.665 120.508 28.035 0.633 1 Pc
ENST00000462776 S100A6-203 0.479 13.011 4.239 0.487 2 Pc
- - 90.06 21.49 0.62 - -

All RNA species of TRAM1 and S100A6 detected in the transcriptome data were listed and TPM and TSL values as well as the exon-intron structure of individual transcripts was analyzed. Primer pairs were designed according to material and methods and RNA species were checked for a specific amplification. Only transcripts marked in bold letters are targeted by both primers and result in a finally calculated lg(fc) value for a potential new biomarker. (Legend: Protein coding (Pc), Retained Intron (Ri), Processed transcript (Pt)).