Table 4. Differences in gene expression of selected markers in ECV-304 and RT-4 cells compared to the results of urine pools C and HR of RNA-Seq.

Transcript	HR [TPM]	C [TPM]	lg(HR/C)	ECV-304	RT-4	lg(ECV-304/ RT-4)
CXCL8	382.32	93.83	0.61	111.86	181.13	-0.21
RPS27	395.04	133.20	0.47	149.51	81.14	0.27
RPL23A	82.19	24.97	0.52	327.93	383.14	-0.07
TRAM1	4.28	12.23	-0.46	35.83	143.36	-0.60
S100A6	90.06	21.49	0.62	1027.96	281.88	0.56

Shown are the TPM and lg(fc) values of the high risk (HR) and the control (C) patient pool for five potential biomarkers, which were previously analyzed via RNA-Seq. These selected marker candidates were also analyzed in bladder cancer cell lines (ECV-304 and RT-4) via RT-qPCR (see Tables 2 and 3). All data was normalized to the expression of 18S rRNA and used for calculation of lg(fc) values.