Figure 6.

STAT3/miR-204/TRPML1 axis mediates the development of AD by mediating mitochondrial autophagy and ROS production in vivo

(A) Expression of TRPML1 and miR-204 in hippocampus of normal and APP/PS1 mice detected by qRT-PCR. (B) Expression of STAT3/p-STAT3 normalized to β-actin in hippocampus of mice in normal and APP/PS1 mice detected by western blot analysis is shown. (C) Expression of mitochondrial-autophagy-related proteins normalized to β-actin in hippocampus of normal and APP/PS1 mice detected by western blot analysis is shown. (D) Frequency of mitochondrial autophagy in hippocampus of mice in each group observed by electron microscopy is shown. (E) ROS production in hippocampus mitochondria of mice in each group detected by MitoSOX fluorescence is shown. MitoSOX was oxidized to generate red fluorescence, and the nucleus was stained in blue by DAPI. (F) Escape latency in the Morris water maze test is shown. (G) The time spent in the quadrant containing the hidden platform in the Morris water maze test is shown. (H) The times of crossing the maze platform detected by water maze test are shown. ∗p < 0.05. Measurement data were expressed in the form of mean ± standard deviation. The comparison between multiple groups was conducted by one-way ANOVA, followed by Tukey’s post-test, and the comparison between multiple groups at different time points was conducted by repeated-measurement ANOVA, with Bonferroni’s post-test. n = 5.