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. 2021 May 10;16:3217–3240. doi: 10.2147/IJN.S302450

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© 2021 Zhang et al.

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Selection and evaluation of GD2 aptamer. (A) In vivo SELEX process. DNA pool was administered through tail vein injection to GD2⁺ tumor-bearing mice. DNA pool could distribute rapidly ssDNA were extracted and amplified by PCR. PCR products were preceded to next selection part. (B) Flow cytometry monitoring of selection effect. Selected pool of each round was amplified and labeled by FAM, and further incubated with GD2-coated beads. Fluorescence signal was assessed. (C) Flow cytometry assessment of aptamer for GD2 binding specificities. FAM-DB67 was incubated with GD2⁺ cells IMR32 and GD2^– cells A431, respectively. Fluorescence signal was assessed.