Table 1.

Primer Sequences Used for the Quantitative Real-Time PCR

Gene	Forward Primer Sequence (5ʹ-3ʹ)	Reverse Primer Sequence (5ʹ-3ʹ)
ALP	CCAACTCTTTTGTGCCAGAGA	GGCTACATTGGTGTTGAGCTTTT
Runx2	GGACGAGGCAAGAGTTTCA	TGGTGCAGAGTTCAGGGAG
OPN	TGGATGAACCAAGCGTGGA	TCGCCTGACTGTCGATAGCA
OCN	GCCCTGACTGCATTCTGCCTCT	TCACCACCTTACTGCCCTCCTG
GAPDH	TGCACCACCAACTGCTTAGC	GGCATGCACTGTGGTCATGAG

Abbreviations: PCR, polymerase chain reaction; ALP, alkaline phosphatase; Runx2, runt-related transcription factor 2; OPN, osteopontin; OCN, osteocalcin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.