Table 2.

Specific activity of full-length SdGH5_8-CBM10x3 and the three truncated forms on soluble (CGM-lv, high-viscosity CGM, KGM, and GG) and insoluble (INM) mannose-containing polysaccharides and the insoluble Avicel (see Fig. 1 for polysaccharide structures)

Substrates	Units	SdGH5_8-CBM10x3	SdGH5_8-CBM10x2	SdGH5_8-CBM10x1	SdGH5_8 (CD alone)
CGM-lv	U/mg	1972 ± 80	2285 ± 150	1539 ± 88	2906 ± 53
	(μmol/s)/μmol proteina	1729 ± 70	1763 ± 116	1046 ± 60	1690 ± 31
	Relative (%)b	100	102	60	98
CGM (high viscosity)	U/mg	2212 ± 78	2755 ± 109	1814 ± 292	3151 ± 304
	(μmol/s)/μmol proteina	1939 ± 69	2126 ± 84	1234 ± 198	1833 ± 177
	Relative (%)b	100	110	64	95
KGM	U/mg	3544 ± 110	3617 ± 112	5136 ± 128	4556 ± 108
	(μmol/s)/μmol proteina	3107 ± 97	2791 ± 87	3493 ± 87	2650 ± 63
	Relative (%)b	100	90	112	85
GG	U/mg	40 ± 8	24 ± 1	200 ± 33	108 ± 25
	(μmol/s)/μmol proteina	35 ± 7	19 ± 1	136 ± 23	63 ± 14
	Relative (%)b	100	54	389	180
INM	U/mg	9 ± 1	38 ± 3	106 ± 6	81 ± 7
	(μmol/s)/μmol proteina	8 ± 1	29 ± 2	72 ± 4	47 ± 4
	Relative (%)b	100	363	900	588
Avicel	U/mg	NDc	NDc	NDc	NDc

ND, not determined.

^aSpecific activity calculated based on molecular weight.

^bSpecific activity relative to full-length SdGH5_8-CBM10x3 calculated based on the specific activity given in (μmol/s)/μmol protein.

^cNo activity detected.