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Binding to CGM-lv analyzed by AGE
| Protein form | Kd (mg/ml) |
|---|---|
| SdGH5_8-CBM10x3 | <0.125a |
| SdGH5_8-CBM10x2 | No binding |
| SdGH5_8-CBM10x1 | No binding |
| SdGH5_8 (no CBMs) | No binding |
| SdGH5CBM10x3_GFP | <0.125a |
| SdGH5CBM10-1_GFP | 3.7 |
| SdGH5CBM10-2_GFP | 0.4 |
| SdGH5CBM10-3_GFP | 0.2 |
| GFP | No binding |
Saturation was reached already at the lowest in-gel galactomannan concentration.
