Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
| Volume (μL) | Reagent |
|---|---|
| 27.5 | Water |
| 2.0 | gDNA (100 ng) |
| 10.0 | 5× buffer Long Range (no MgCl2) |
| 3.5 | MgCl2 (25 mM) |
| 1.5 | dNTPs (10 mM) |
| 0.5 | KAPA LR enzyme |
| 2.5 | F primer (10 μM) |
| 2.5 | R primer (10 μM) |
| Temperature (°C) | Time (s) | # cycles |
|---|---|---|
| 94 | 180 | |
| 94 55∗ 68 |
15 15 Allow 1 min/kb |
10 |
| 94 63∗ 68 |
15 15 Allow 1 min/kb |
25 |
| 72 | 10 min | |
| 4 | hold |
