| Volume (μL) | Reagent |
|---|---|
| 75 ng | PCR product |
| 75 ng | Nanotag reporter vector |
| 2.0 | T4 DNA ligase buffer |
| 1.0 | T4 DNA ligase (NEB, M0202) |
| 1.0 | BsmBI (NEB, R0508) |
| Up to 20 μL | Water |
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