Fig. 6.

Acrp30 and leptin exert antagonizing effects on BCPAP cell migration. A scraped wound was inflicted on a confluent monolayer of BCPAP cells treated with the indicated doses of Acrp30, with leptin, alone, or in combination. Immediately following the scratch (0 h), after 8 and 30 h, cells were photographed under microscopy (a), and the closure of the gap was measured by calculating pixel densities in the wound area and expressed as percentage of wound closure ± standard error of the mean (SEM) (b). The statistical analysis was evaluated using the one-way ANOVA test. *p < 0.05; **p < 0.01; ***p < 0.001 versus NC