Wash media
| Components | Final concentration | Amount |
|---|---|---|
| DMEM/F-12 | n/a | 48 mL |
| B27 without vitamin A | 1× | 1 mL |
| GlutaMAX | 1× | 500 μl |
| Antibiotic-Antimycotic | 1× | 500 μl |
| Total | n/a | 50 mL |
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