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Neuron/Astrocyte differentiation media:
| Components | Final concentration | Amount |
|---|---|---|
| Neural Stem Cell Basal Medium | n/a | ~47.9 mL |
| B27 without vitamin A | 1× | 1 mL |
| GlutaMAX | 1× | 500 μl |
| Antibiotic-Antimycotic | 1× | 500 μl |
| Forskolin | 1 μM | 50 μl |
| Retinoic acid | 1 μM | 50 μl |
| Total | n/a | 50 mL |
