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. 2021 May 4;2(2):100522. doi: 10.1016/j.xpro.2021.100522

OPC media

Components Final concentration Amount
DMEM/F12 n/a ~44.95 mL
GlutaMAX 500 μl
B27 without vitamin A 1 mL
N2 supplement 500 μl
apo-Transferrin 50 μg/mL 500 μl
Biotin 10 nM 500 μl
Bovine serum albumin 0.1% 500 μl
Hydrocortisone 10 nM 500 μl
Insulin 5 μg/mL 50 μl
PDGF-AA 10 ng/mL 500 μl
Sodium selenite 30 nM 500 μl
Total n/a 50 mL