Components | Final concentration | Amount |
---|---|---|
DMEM/F12 | n/a | ~44.95 mL |
GlutaMAX | 1× | 500 μl |
B27 without vitamin A | 1× | 1 mL |
N2 supplement | 1× | 500 μl |
apo-Transferrin | 50 μg/mL | 500 μl |
Biotin | 10 nM | 500 μl |
Bovine serum albumin | 0.1% | 500 μl |
Hydrocortisone | 10 nM | 500 μl |
Insulin | 5 μg/mL | 50 μl |
PDGF-AA | 10 ng/mL | 500 μl |
Sodium selenite | 30 nM | 500 μl |
Total | n/a | 50 mL |