Fig. 2. miR-135b enhances growth and proliferation of NSCLC cells in vitro.

A, B CCK-8 assay analysis of cell growth in the indicated stable cell lines. C, D Representative pictures (Left panel) and quantification (Right panel) of cell colony formation. E, F Representative pictures (Upper panel) and quantification (lower panel) of EdU staining. EdU was labeled with TRITC (red) and cells were counterstained with DAPI (blue). IN-NC: Inhibiting negative control; 135b-IN: miR-135b inhibiting. Scale bars, 20 μm. Error bars represent the mean±SD obtained from three independent experiments. p values are calculated by a two-tailed, unpaired t-test. *p < 0.05; **p < 0.01; ***p < 0.001.