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. 2021 May 14;12(5):493. doi: 10.1038/s41419-021-03773-x

Fig. 8. IL-6/STAT3 transactivates the expression of miR-135b in NSCLC.

Fig. 8

A Real-time PCR measuring the expression of miR-135b in H292 and A549 cells treated with human recombinant IL-6 (10 ng/ml, 6 h). B, C Schematic illustration of the arrangement of the promoter region of miR-135b and the top 5 conserved STAT3-binding sites predicted by the JASPAR database. D ChIP-PCR assay analyzing the enrichment of STAT3 and H3K4Me3 in the promoter of miR-135b. E Schematic illustration of the construction of miR-135b promoter luciferase reporters containing a region around site #1 with the STAT3 binding site intact (WT) or mutated (Mut). F The effects of STAT3 on the activity of the WT or Mut miR-135b promoter-luciferase reporter in 293FT cells. G Schematic representation of the STAT3/miR-135b/NF-κB signaling that promoting aggressiveness in NSCLC.