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. 2021 May 14;9:24. doi: 10.1038/s41413-021-00136-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Depletion of NRP2 escalates the resorptive function and gene expression in osteoclasts. Knockout of NRP2 in osteoclastic precursors isolated from NRP2 Fl/Fl; CSF1R-Cre transgenic mice by addition of 4-HydroxyTamoxifen in vitro and differentiated into osteoclasts in the presence of RANKL and M-CSF, LNCaP C4-2B CM and PC3 CM on a 24-well osteoassay plate. a–c Representative images of pit resorption to compare the resorbed area in NRP2WT and NRP2KO osteoclasts in RANKL+M-CSF, LNCAP C4-2B CM and PC3 CM respectively. d–f Quantification and comparison of the percentage resorbed area in NRP2WT and NRP2KO osteoclasts in RANKL and M-CSF, LNCaP C4-2B CM and PC3 CM respectively. g–i Graphical representation of the osteoclastic gene expression at mRNA in NRP2WT and NRP2KO condition treated respectively with RANKL and M-CSF, LNCaP C4-2B CM and PC3 CM. All values reported as mean± SEM from three independent experiments and represented as a bar graph with error bar. Statistically significant P value denoted as * (0.05), ** (0.01), *** (0.001)