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. 2021 May 14;9:24. doi: 10.1038/s41413-021-00136-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Cytokine analysis of CM collected from LNCaP C4-2B and PC3. Graphical representation of absolute a RANKL, b M-CSF and c GM-CSF concentration in pg·mL⁻¹ in LNCaP C4-2B and PC3 CM by ELISA. d Osteoclastic precursors in NRP2WT and NRP2KO condition treated with PC3 CM without and with RANKL at 50 ng·mL⁻¹ and graphically represented in g. e Human cytokine array membrane containing 36 different cytokines were incubated with CM from LNCaP C4-2B and PC3 mixed with biotynylated detection antibodies. The bound cytokine developed with Streptavidin-HRP and chemiluminescent detection reagents. Light produced at each spot is proportional to the amount of cytokine bound. Dot blot showing the presence of cytokine in LNCaP C4-2B CM (left) and PC3 CM (right) along with the table showing the complete list of cytokines that were found in the CM. f Average pixel density of each cytokine observed in the dot blots was analyzed by Imagej software and graphically represented to compare the cytokines obtained in LNCaP C4-2B and PC3 CM