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. 2021 May 14;12(5):490. doi: 10.1038/s41419-021-03771-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Cell extracts from H460 cells treated with CPM under hypoxia were immunoprecipitated with anti-APEX1 antibody or IgG. The IP complex was analyzed for HIF-1α or APEX1 by western blotting. B H460 cells were treated with CPM under hypoxia. Intracellular localization of APEX1 and HIF-1α was examined by immunofluorescence. APEX1 (green), HIF-1α (red), DAPI (blue) staining, and 3-channel merged images indicated the nuclear colocalization. C H460 and A549 cells were treated with E3330 under normoxia and hypoxia. D H460 and A549 LC cells were treated with LW6 under normoxia and hypoxia. E LC cells H460 and A549 treated with CPM under normoxia and hypoxia. Cells were stained with 10 μM DCFH-DA and measured with a flow cytometer.