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. 2021 May 14;12(5):490. doi: 10.1038/s41419-021-03771-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Western blot analysis of CA9 expression at various concentrations treated with CPM. B Intracellular pH determination of H460 cell line treated with APEX1 inhibitor E3330 under normoxia and hypoxia using flow cytometry. C Intracellular pH determination of H460 cells line treated with LW6 under normoxia and hypoxia using flow cytometry. D Intracellular pH determination of H460 cell line-treated CPM under normoxia and hypoxia using flow cytometry, using BCECF-AM dye for staining in H460 cells, images taken by a fluorescence microscope.