Table 3.
MLST primer sets for Leptospira borgpetersenii clone FU18027.
| Locus | PCR | Primer name | Sequence (5′–3′) | References |
|---|---|---|---|---|
| glmU | 1st | glmU-FM | AGGATAAGGTCGCTGTGGTA | 41 |
| 1st | glmU-RM | AGTTTTTTTCCGGAGTTTCT | 41 | |
| 2nd | 1-glmU-2F_M13 | TGTAAAACGACGGCCAGTCGYATGAAAACGGATCAG | 42 | |
| 2nd | 1-glmU-2R_M13 | CAGGAAACAGCTATGACCGGAAGRTARTATTCDCCCTG | 42 | |
| pntA | 1st | pntA-F-borg | GCCGGAGCAAATCTTGTATC | This study |
| 1st | pntA-R-borg | TGACCGATTACCGTTACCCC | This study | |
| sucA | 1st | sucA-FM | TCATTCCACTTYTAGATACGAT | 41 |
| 1st | sucA-RM | TCTTTTTTGAATTTTTGACG | 41 | |
| 2nd | 3-sucA-2F_M13 | TGTAAAACGACGGCCAGTGCSGGTRATCATCWBATGG | 42 | |
| 2nd | 3-sucA-2R_M13 | CAGGAAACAGCTATGACCGRAAWCCYTTYGCAAGATC | 42 | |
| tpiA | 1st | tpiA-F-borg | AAATCGCATGCGCAAAACGG | This study |
| 1st | tpiA-R-borg | GAGCGCTTGTATGTTATCCG | This study | |
| 2nd | tpiA-F2-borg | CGCCGGAAACTGGAAAATGA | This study | |
| 2nd | tpiA-R2-borg | TTCCGCAATTTCTTTCGCGC | This study | |
| pfkB | 1st | pfkB-F-borg | CCGGGAAGGTTTCCAAAGAC | This study |
| 1st | pfkB-R-borg | TAAAACCGTGGGTCAGTCCG | This study | |
| 2nd | pfkB-F2-borg | GGAAAACGCCGGAATCCTTT | This study | |
| 2nd | pfkB-RM | AGAACACCCGCCGCAAAACAAT | 41 | |
| mreA | 1st | mreA-F-borg | GGTGGAAAGATATGGCTCGC | This study |
| 1st | mreA-R-borg | TTCCTATCGCGGTCATGGAC | This study | |
| caiB | 1st | caiB-F | CAACTTGCGGAYATAGGAGGAG | 41 |
| 1st | caiB-R-borg | TCCGAGAGATCGGTAAATCG | This study | |
| 2nd | 7-caiB-2F_M13 | TGTAAAACGACGGCCAGTCTTKCTTCRATYTTGGCG | 42 | |
| 2nd | 7-caiB-2R_M13 | CAGGAAACAGCTATGACCAMCGATATGTWAYMGGRGTT | 42 | |
| glmU | 1st | glmU-FM | AGGATAAGGTCGCTGTGGTA | 41 |
| 1st | glmU-RM | AGTTTTTTTCCGGAGTTTCT | 41 | |
| 2nd | 1-glmU-2F_M13 | TGTAAAACGACGGCCAGTCGYATGAAAACGGATCAG | 42 | |
| 2nd | 1-glmU-2R_M13 | CAGGAAACAGCTATGACCGGAAGRTARTATTCDCCCTG | 42 |
MLST, multilocus sequence typing.