Table 3.

Summary of differences in relative expression or fold increase across STAT1 mutants compared with wild type under various conditions.

Mutation	Condition/stimulation	GBP1	IFIT2	IRF1	APOL6	OAS1
E235G	Baseline (full serum)^a	↑	NS	NS	↑	NS
	Serum starvation^b	↑	NS	NS	↑	NS
	IFNα^c	NS	↑	↑	NS	NS
	IFNγ^d	↓	NS	NS	↓	↑
K278E	Baseline (full serum)^a	NS	NS	NS	NS	NS
	Serum starvation^b	NS	NS	NS	NS	NS
	IFNα^c	↓	NS	NS	↓	↓
	IFNγ^d	NS	↑	NS	NS	NS
P329L	Baseline (full serum)^a	NS	NS	NS	↑	↑
	Serum starvation^b	NS	NS	↑	↑	↑
	IFNα^c	↓	NS	NS	↓	↓
	IFNγ^d	↓	NS	NS	↓	NS
T385M	Baseline (full serum)^a	NS	NS	NS	NS	NS
	Serum starvation^b	NS	NS	NS	NS	NS
	IFNα^c	↑	↑	↑	↑	↑
	IFNγ^d	NS	NS	NS	↓	NS
D517G	Baseline (full serum)^a	NS	↑	NS	NS	NS
	Serum starvation^b	NS	NS	NS	NS	NS
	IFNα^c	NS	NS	NS	↓	↓
	IFNγ^d	NS	NS	NS	↓	NS
Y701C	Baseline (full serum)^a	NS	NS	NS	NS	↓
	Serum starvation^b	NS	NS	NS	NS	NS
	IFNα^c	↓	↓	↓	↓	NS
	IFNγ^d	↓	↓	↓	↓	NS

NS not significant.

^aRelative gene expression compared with wild type under full-serum culture conditions without cytokine stimulation.

^bRelative gene expression compared with wild type following 24 h of low-serum conditions without cytokine stimulation.

^cFold increase in gene expression from baseline compared with wild type following 6 h of stimulation with IFNα (10 ng/mL).

^dFold increase in gene expression from baseline compared with wild type following 6 h of stimulation with IFNγ (10 ng/mL).