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. 2021 May 14;4:572. doi: 10.1038/s42003-021-02038-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — To evaluate Treg cells, uterus-draining para-aortic lymph nodes (PALN) were collected from female mice in estrus (unmated control, con), or on day 3.5 pc after mating with either intact (int) or vasectomized (vas) males. CD4⁺ T cells were analyzed by flow cytometry for CD4, FOXP3 and NRP1 expression, and the numbers of CD4⁺ T cells (A), CD4⁺FOXP3⁺NRP1^−/lo peripheral Treg cells (B), CD4⁺FOXP3⁺NRP1⁺ thymic Treg cells (C), and total CD4⁺FOXP3⁺ Treg cells (D) were quantified. Symbols depict data from individual mice (n = 10–13/group) and mean ± SEM values are shown. Data were analyzed by one-way ANOVA with post-hoc Sidak t-test, or non-parametric Kruskal Wallis test with post-hoc Dunn’s multiple comparisons test. ^#P < 0.05 compared to unmated estrus control group, *P < 0.05 compared to vasectomized-mated group.