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. 2021 May 14;12(5):491. doi: 10.1038/s41419-021-03783-9

Fig. 1. Reduction of CG6015 in spermatogonia led to accumulation of undifferentiated cells and elongated spermatozoon defects.

Fig. 1

a Relative mRNA level of CG6015 in the W1118, Bam>CG6015 RNAi and Bam>CG6015 RNAi; Δ86/+ testes. b Whole mount DNA staining of the W1118, Δ86/+ , Bam>CG6015 RNAi and Bam>CG6015 RNAi; Δ86/+ testes. Yellow arrows represent undifferentiated cells at the apex of the testis. c The distance of deep stained nuclei at the apex of W1118 (n = 50), Δ86/+ (n = 27), Bam>CG6015 RNAi (n = 36) and Bam>CG6015 RNAi; Δ86/+ (n = 16) testes. d Elongated spermatid clusters at the tail of testes. d’ Enlargements of corresponding elongated spermatid clusters. e The number of elongated spermatid clusters in W1118 (n = 44), Δ86/+ (n = 25), Bam>CG6015 RNAi (n = 33) and Bam>CG6015 RNAi; Δ86/+ (n = 10) testes. DNA was stained with Hoechst (blue). ***P < 0.001, ns. not significant. Scale bar: 50 μm for b; 20 μm for d and d’.