Fig. 2. Reduction of CG6015 in spermatogonia restrained germline differentiation.

a–d Immunostaining of Vasa (red) and 1B1 (green) at the apex of W¹¹¹⁸, Δ86/+, Bam>CG6015 RNAi and Bam>CG6015 RNAi; Δ86/+ testes. e The number of punctate fusomes (also defined as ‘unbranched fusomes’ among germ cells) at the apex of W¹¹¹⁸ (n = 13), Δ86/+ (n = 12), Bam>CG6015 RNAi (n = 11) and Bam>CG6015 RNAi; Δ86/+ (n = 13) testes. f The number of branched fusomes at the apex of W¹¹¹⁸ (n = 13), Δ86/+ (n = 12), Bam>CG6015 RNAi (n = 11) and Bam>CG6015 RNAi; Δ86/+ (n = 13) testes. g–i Immunostaining of FasIII (red), 1B1 (red) and Bam-GFP (green) at the apex of Δ86/+ , Bam>CG6015 RNAi and Bam>CG6015 RNAi; Δ86/+ testes. j Fluorescence intensity of Bam-GFP at the apex of Δ86/+ (n = 15), Bam>CG6015 RNAi (n = 3) and Bam>CG6015 RNAi; Δ86/+ (n = 3) testes. DNA was stained with Hoechst (blue). ***P < 0.001, ns. not significant. Scale bars: 20 µm.