Fig. 3. Reduction of CG6015 in spermatogonia increased proliferation in Drosophila testes.

a–d Immunostaining of PH3 (red) and FasIII (green) at the apex of W¹¹¹⁸, Δ86/+ , Bam>CG6015 RNAi and Bam>CG6015 RNAi; Δ86/+ testes. DNA was stained with Hoechst (blue). Yellow lines represented the distance between PH3 positive cells and hub cells. e The number of PH3 positive cells in W¹¹¹⁸ (n = 12), Δ86/+ (n = 9), Bam>CG6015 RNAi (n = 10) and Bam>CG6015 RNAi; Δ86/+ (n = 10) testes. f The distance between PH3 positive cells and hub cells in W¹¹¹⁸ (n = 12), Δ86/+ (n = 9), Bam>CG6015 RNAi (n = 11) and Bam>CG6015 RNAi; Δ86/+ (n = 10) testes. ***P < 0.001, ns. not significant. Scale bars: 20 µm.