Fig. 6. Reduction of CG6015 in spermatogonia upregulated dpERK in GSC-like cells.

a–c Immunostaining of dpERK (green) and Vasa (red) at the apex of W¹¹¹⁸, Bam>CG6015 RNAi, and Bam>CG6015 RNAi; Δ86/+ testes. Representative germline dpERK signals are shown by using yellow arrowheads. a’–c’ Enlargements of the immunostaining of dpERK and Vasa signals. d–g Immunostaining of dpERK (red) and 1B1 (green) at the apex of W¹¹¹⁸, Δ86/+, Bam>CG6015 RNAi and Bam>CG6015 RNAi; Δ86/+ testes. d’–g’ Enlargements of immunostaining of dpERK and 1B1 signals. DNA was stained with Hoechst (blue). Scale bars: 20 µm.