Table 4.

Experimental design and results of central composite design (CCD).

Run order	Concentration	Temperature	Carbon	Inoculum	Response
					Experimental	Predicted
1	100	35	1.5	50005000	71.1	71.2
2	100	45	1.5	50005000	30.2	38.1
3	75	30	3	100000000	80.1	73.6
4	125	40	3	10000	22.4	18.6
5	50	35	1.5	50005000	91.2	87.6
6	75	30	3	10000	22.3	30.6
7	125	40	0	10000	15.4	14.5
8	100	35	− 1.5	50005000	11.2	17.3
9	125	40	0	100000000	35.4	28.5
10	100	35	1.5	50005000	71.5	71.2
11	75	40	0	10000	37.7	43.6
12	100	35	1.5	50005000	71.9	71.2
13	125	30	3	10000	11.1	12.0
14	125	30	3	100000000	65.7	55.5
15	125	40	3	100000000	69.2	57.1
16	100	35	1.5	50005000	69.8	71.2
17	75	40	0	100000000	65.1	57.1
18	125	30	0	100000000	33.4	23.8
19	100	35	1.5	50005000	71.6	71.2
20	75	40	3	100000000	90.2	74.3
21	100	25	1.5	50005000	25.4	27.8
22	100	35	1.5	− 49985000	6.2	3.4
23	75	40	3	10000	34.1	36.3
24	150	35	1.5	50005000	30.1	40.3
25	100	35	1.5	149995000	45.2	56.9
26	125	30	0	10000	3.4	4.8
27	100	35	1.5	50005000	71.5	71.2
28	75	30	0	100000000	60.2	53.5
29	75	30	0	10000	30.2	35.0
30	100	35	4.5	50005000	30.4	41.6
31	100	35	1.5	50005000	71.3	71.2