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. 2021 May 14;11:10351. doi: 10.1038/s41598-021-89603-8

Figure 3.

Figure 3

Proliferative ability of DMD-iPSC-CMs. (A) Troponin T-positive cells was identified using immunofluorescence staining in Con-iPSC-CMs, DMD-iPSC-CMs, and Ed-DMD-iPSC-CMs, on days 1 and 4 post seeding (scale bar 200 µm). (B) Number of troponin T-positive cells according to the time course from day 1 to day 4 (n = 4 sessions). (C) Proliferation rate was evaluated in Con-iPSC-CMs, DMD-iPSC-CMs, and Ed-DMD-iPSC-CMs (n = 4 sessions, mean ± SD, *P < 0.05, N.S. not significantly different). (D) Ki67 expression was detected using immunofluorescence staining in Con-iPSC-CMs, DMD-iPSC-CMs, and Ed-DMD-iPSC-CMs (scale bar 50 µm). Arrowheads indicate Ki67-positive cells in troponin T-positive cells. (E) Percentage of Ki67-positive cells in troponin T-positive cells was evaluated in Con-iPSC-CMs, DMD-iPSC-CMs, and Ed-DMD-iPSC-CMs (n = 4 sessions, mean analyzed troponin T-positive cell number = 1974 ± 651, 1560 ± 971, and 3512 ± 3547 cells, respectively, mean ± SD, *P < 0.05, N.S. not significantly different).