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Identification of an acrolein-specific IgM–BCR.A, the hybridoma clones showing a specificity toward acrBSA. The PerC cells were fused with P3U1 mouse myeloma cells and, after screening based on the specific binding to acrBSA, 36 hybridoma clones were obtained. B, specificity of IgM mAb produced by hybridoma clone RE-G25 (clone No. 25). C, VDJ gene usage of VH for RE-G25. acrBSA, acrolein-modified BSA; BCR, B cell receptor; IgM, immunoglobulin M.
