Comparison of the usage of IGHV, IGHD, and IGHJ genes of IgM–BCRs in ACR+and ACR−PerC B cell populations. The PerC B cells isolated from BALB/c mice were fractionated into the ACR+ and ACR− B cell populations by flow cytometry. The mean percentage usages of IGHV, IGHD, and IGHJ are shown. The bars indicate the mean percentage usage of ACR+ (blue) and ACR− (gray) B cells. A, IGHV gene usage. B, IGHD gene usage. C, IGHJ gene usage. D, usages of the IGHV5 gene segments. ACR−, acrolein-nonbinding; ACR+, acrolein-binding; BCR, B cell receptor; IGHD, immunoglobulin heavy-chain diversity; IGHJ, immunoglobulin heavy-chain joining; IGHV, immunoglobulin heavy-chain variable; IgM, immunoglobulin M.