Fig. 6.

HA attenuated acute oxidative insult in H9c2 cells in an O-GlcNAcylation-dependent manner. A, The global cellular reactive oxygen species (ROS) production measured in H9c2 cells measured by CM-H2DCFDA (n = 6–7 per group). B, Representative fluorescence images of oxidative DNA damage measured by immunostaining for 8-Oxo-dG (red), nuclei (DAPI; blue) and mitochondria (Tom20; green). Scale bar, 10 μm. C, Quantitative analysis of 8-Oxo-dG positive staining in nuclei and mitochondria, and the results were normalized to the intensity in nuclear compartment of the control group (n = 50 cells per group). D, The MitoSOX flow cytometry analysis and the fluorescence intensity in H9c2 cells with indicated treatments (n = 3 per group). E, The oxygen consumption rates (OCR) of H9c2 cells at baseline and after FCCP stimulation (n = 5–6 per group). F, The levels of apoptosis in H9c2 cells with indicated treatments (n = 5 per group). Data are shown as mean ± SEM. *P < 0.05, **P < 0.01 and ***P < 0.001 for indicated comparisons. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)