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. 2021 May 6;2(2):100518. doi: 10.1016/j.xpro.2021.100518

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© 2021 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Isolated adult mouse microglia have a low proliferative capacity in vitro

Representative fluorescent images of microglial cultures following the addition of Edu for 48 h (A). White arrowheads represent PU.1 positive microglia, whilst white arrows represent PU.1⁺ Edu⁺, dividing microglia. Red arrowheads represent PU.1^- cells, whilst red arrows represent PU.1^- Edu⁺ dividing non-microglial cells, which are largely pericytes. Scale bar, 50 μm. Average percentage of Edu+ microglia (PU.1⁺) per case, n = 6 adult mouse brain isolations (B). All data are plotted as mean ± SEM.