Skip to main content
. 2021 May 6;2(2):100518. doi: 10.1016/j.xpro.2021.100518

Enzyme digestion mix

Reagent Final Concentration Amount
Lyophilized Papain dissolved in 5 mL EBSS (20 U/mL) 2.5 U/ mL 1.25 mL
DNase (1000 U/ mL) 10 U/ mL 100 μL
Hibernate A Medium n/a 8.65 mL
Total n/a 10 mL